Genomic analyses revealed the presence of one complete prophage sequence, Фlj6026 (LJP0764-LJP0819), which is integrated next to tRNA loci at ∼900 kbp within the DPC6026 genome.
If they have been integrated next to other related regulatory proteins or transcriptional factors, their selection might have been favored [ 55].
Only a minority of these prophages (51) integrated next to tRNAs, as is common in other clades (Williams 2002), even if all identified phage integrases were tyrosine recombinases.
For instance, in gene therapy trials using γ-RVs to treat SCID-X1 immunodeficiency, multiple patients developed T-cell acute lymphoblastic leukemia that was directly attributable to the RV randomly integrating next to, and activating the LMO-2 oncogene.
The colony color assay utilizes an ADE2 reporter integrated next to the right telomere of chromosome V. Reporter strains were plated onto YPD medium, incubated for 2 days, and then transferred to 4°C until the color development was sufficient for photometric detection.
Maybe some geeky websites will integrate it next to Facebook Connect and Sign in with Twitter as another way to sign into the service.
It is often desirable to sequester cells in specific locations on the surface and to integrate sensing elements next to the cells.
These samples may have integrated the endonuclease gene next to an endogenous promoter or enhancer such that the endonuclease was expressed in the absence of induction.
We used a HT1080 cell line carrying a LacO array integrated on chromosome 13p next to the NOR and expressing GFP fused to Lac repressor (Chubb et al., 2002).
We first predicted the protein protein interaction in Arabidopsis thaliana with methods, including ortholog, SSBP, gene fusion, gene neighbor, phylogenetic profile, coexpression, protein domain, and used Naïve Bayesian approach next to integrate the results of these methods and text mining data to build a genome-wide protein interactome network.
