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We sequenced and mapped the GLOBE integration sites in the human cells to assess the risk of integration in potentially dangerous loci and showed that GLOBE integrates at low copy number into the human genome without preferential targeting of proto-oncogenes, tumor-supressor or cell cycle related genes.
Because HIV DNA integrates at many sites in the human genome, the site of integration can be used to identify clonally expanded cells that arose from a single infected progenitor.
An antibiotic resistance gene, hygromycin phosphotransferase (HPT), was also present to allow selection of cells with integrated plasmid DNA in media containing hygromycin B. If the pHRT construct integrates at a telomere as a single copy, the CMV promoter will be unable to drive expression as it is located downstream of the RFP sequence.
For retailers, Yoyo integrates at the point of sale to provide information and intelligence the buying habits of its customers.
The multi-functional hand-held device 100 integrates at least two devices 102 into a single device.
miRanalyzer integrates at least 8 features, such as the secondary structure, to train five random forest models for the prediction of novel miRNA candidates.
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We were able to really integrate at the data integration, at the business process level".
Some of the gaps noted were as follows: - Safe motherhood services were not integrated at all health facilities (67.5% integration).
Interestingly, the DNA introduced in the six transformants was integrated at the same locus indicative for a hotspot for integration.
This plasmid was sequence verified and integrated at the RAP1 genomic locus in HKy639 selecting Ura+ integrants (HKy754, HKy755).
Then we work at the N.C.A.A. basketball regional, long since integrated at every level.
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