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The majority of our participants' proviruses were integrated within genes (425/534, 79.6%) (Fig. 1, A to C), particularly within introns (337/425, 79.1%), as previously observed (9, 17, 20).
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In pre-infusion T cells, 51.1% of the proviruses integrated within 4,374 genes were in forward transcriptional orientation, a virtually random distribution (p = 0.03).
In this study, we applied an alternative strategy, focusing on how temporal control of gene expression is integrated within a gene regulatory network.
A novel bivalent chromatin domain is formed at promoter-less artificial CGI-like sequences integrated within a gene desert in mouse ESCs.
The Animal Model module is seamlessly integrated within the gene portal so that the data can be searched and retrieved using a single search engine.
Use of junction fragments containing viral U3 and adjacent cellular sequences, permitted to establish that in one of the most developed tumor one of the proviral genome was integrated within a gene that is known as ccn3 and that we originally designated "nov" for « nephroblastoma overexpressed » [ 5].
High throughput genome sequencing analysis for the characterization of these transformants established that transposon sequences are integrated within the omp10 gene of the A. marginale chromosome and its mobilization within this gene was mediated by the transposase in a cut and paste mechanism, since i). the transposon sequences were integrated within a TA dinucleotide site ii).
As expected, all RV vectors showed a preference for integration around TSSs, while LV vectors integrated preferentially within genes, as compared to the control sequence set (Figure 1).
A mouse ES cell line (ESKN92) generated by gene-trap mutagenesis in which a β-galactosidase-neomycin phosphotransferase (βgeo) reporter cassette integrated within the GR gene, generating a translational fusion between GR and βgeo, has been described previously (23).
Old CfERVs were found mostly in antisense orientation relative to chromosomal genes when integrated within 70 80 kb from the genes (see Fig. 2E).
Additional analysis using longer reads obtained on the Roche/454 platform revealed that the Himar1 transposon was integrated within the omp10 gene.
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