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Plasmids were digested with BstEII to target integration to LEU2, and the number of copies of MPS3 integrated was determined by Southern blotting.
Replication timing of the loci, where tetO and lacO arrays were integrated, was determined as described previously (Kitamura et al., 2006; Saner et al., 2013).
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Prior to integration, the subsets of parent-specific LGs to be integrated were determined by the numbers of commonly mapped markers across parent-specific maps, that is, pairs of LGs showing the largest number of commonly mapped markers were assembled into the same subset.
Point mutations that were successfully integrated were determined by sequencing of genomic DNA purified from the resulting strain.
I knew it had to be fully integrated, and I was determined to stay away from creating one dimension "stances" for each character that are used in bad one person shows to bang the audience over the head with the fact that you are now someone else.
The average integrated intensity per nucleus was determined by using METAMORPH OFFLINE 6.1 software (Universal Imaging, Downingtown, PA, USA), as described previously [ 29].
The fully integrated topology is determined by the position of the loop that undergoes flipping most slowly.
Western blots were scanned and the integrated intensity of each band was determined using Image J Schneiderr et al., 2012).
The number of principal components to be integrated in the linear model was determined using the R package paran [ 25], which is an implementation of the empirical method of Horn's parallel analysis.
Population doubling for CD4+ and CD8+ T cells was determined by integrating CFSE+ peaks using stained, unstimulated cells as a control.
The rolling resistance was determined by integrating the forces acting through the elastic foundation on the wheel axle due to the steady state deformation of the tread band.
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