The importance of the one-spot MD is usually for identifying the least and most visually integrated spots.
ScanArray Lite® microarray scanner was used to scan arrays and ScanArray Express® software (PerkinElmer, Wellesley, MA) was used to process array images, align spots, integrate robot-spotting files with the microarray images and quantify spots as we previously described [ 37].
was then used to process array images, align spots, integrate robot-spotting files with the microarray image, and to export reports of spot intensity data.
Telomere images were captured with a Plan Fluor 100× oil-emersion objective, and individual telomere fluorescence was integrated using spot IOD analysis in the TFL-Telo 2.2 program (gift from Dr. Peter Lansdorp, Vancouver) (Rufer et al., 1998).
For the match set containing images with pI 5-8, the spot volume (intensity integrated over the spot area) was normalized by the volume of total spots in the gel.
For the match set containing images from pI 5 8, the spot volume (intensity integrated over the spot area) was normalized to the total volume of spots in the gel.
Hybridisation signals were quantified using the HDG Analyzer software (Genomic Solutions, Ann Arbo, MI, USA) by integrating all spot pixel signal intensities and removing spot background values determined in the neighbouring area.
Hybridization signals were quantified with the HDG Analyzer software (Genomic Solution, Ann Arbor, MI, USA), by integrating all spot pixel intensities and removing a spot background value determined in the neighboring area.
Similarly Bluefuse integrates various spot quality parameters (eg circularity, uniformity etc) to provide a spot quality assessment.
In this paper, a decision-making methodology is presented which allows some aspects of the robustness issues to be integrated into the spot weld design process.
