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Polyclonal populations of cells harboring integrated sequences were selected with puromycin and tested for overexpression of FLAG-tagged LCN7.
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These integrated sequences will be used to assemble the scaffolds in the B. rapa genome.
In addition to the characterization of circulating plasma virus, integrated provirus sequences were analyzed from two blood cell populations: CD4+ T cells and CD14+ CD16+ monocytes.
Stably integrated eGFP-sequences were used for tracing donor cells in recipients after transplantation.
Briefly, specific Illumina barcode and index sequences were integrated by a second and third PCR.
The three data sets were integrated, while the protein sequences were retrieved from the UniProt database.
The LF82 chromosome and plasmid sequences were integrated into the MicroScope system [87] to perform annotation a comparative analysis with the other E. coli strains published in the context of the ColiScope project [48].
Sequences were integrated at endogenous gene loci following the strategy outlined in [ 42].
The sequences were integrated into ARB and compared to all sequences of the region amplified available in GenBank (Fig. 4b).
In this study, over 1,737 Solexa sequences were integrated in the genetic map through linkage analysis by statistical significance test.
These sequences were integrated with the 212 conserved miRNA sequences identified based on the comparison with A. thaliana miRNA sequences that also passed the structural feature filter.
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