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An integrated quantification of individual patterns of HER-receptor alterations may enable optimised patient stratification with respect to disease outcome.
doi: 10.7554/eLife.03282 Published 5 December 2014 Due to a production error, in the final published article the abstract was given as: The integrated quantification of spontaneous dynamic cell cell force transmissions at both multi-cellular and sub-cellular scales enables spatiotemporal correlations of stress distribution with biomolecules in small cell clusters.
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An additional advantage of the approach lies in the fact that it integrates dyssynchrony quantification of the whole ventricle within the most accurate and reproducible echocardiographic assessment of LV volumes and ejection fraction used to monitor response.
The entire area under the peak encompassing all isomers was integrated for quantification.
For quantification of ethidium uptake, integrated densities of ethidium fluorescence in 20 randomly chosen cells from each micrograph were measured using ImageJ.
This protocol has the advantage that it does not rely on transgene expression in order to quantify the titer of the vector preparation and allows a non-biased quantification of integrating particles.
All clones engrafted with similar efficiency over the first week after implantation, as determined by qRT PCR quantification of the genomically integrated β-gal retroviral copies (Appendix Fig S1).
These were immediately analyzed for pH, pCO2 (standard electrodes), and the plasma concentrations of sodium (Na+), potassium (K+), calcium (Ca2+), magnesium (Mg2+), chloride (Cl-) (ion-selective electrodes), and lactate (Lac-) (enzymatic method, quantification of H2O2), all integrated in a blood gas and electrolyte analyzer (Rapidlab 860; Bayer Diagnostics) and measured at 37°C.
On the basis of analyses and calculations integrating adequate standard curves, the relative quantification of gene expression was determined.
For quantification of fluorescence intensities, the integrated intensity was measured from three areas of fixed size within each embryo and then averaged.
This is the framework within which this project is integrated: the estimation of the prevalence and quantification of the underdiagnosis in the general population.
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CEO of Professional Science Editing for Scientists @ prosciediting.com