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Peaks were integrated individually and the total integrated peak area of each spectrum normalized to 10000.
The average integrated peak area ± SE for 13 selected ion peaks are presented in Figure 2.
Then the integrated peak area versus concentration graph (Fig. 2) was constructed.
Fig. 2 Graph of concentration versus integrated peak area for standard caffeine in water.
The signal was defined as the integrated peak area [18] in an interval of ±250 Hz around the peak.
The atomic composition of Pb, Te, and O is calculated using the integrated peak area and sensitivity factors, and atomic ratio of Pb Te:O is 1 1.52 3.79.
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Further examination of the data revealed that neither noise level nor integrated peak areas were significantly different between PET gantry OFF and ON conditions.
Curve fit parameters were used to calculate the reported integrated peak areas.
The integrated peak areas of top six transitions were calculated using Skyline software (MacLean et al., 2010).
The integrated peak areas for the individual transitions detecting the 4 5 ion fragments per peptide were summed.
Data were analyzed using CasaXPS program, and ratios of integrated peak areas for each individual element were used for quantification.
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