Exact(1)
After sequencing, the constructs were inserted into pUAST attB vector using EcoRI and KpnI whose recognition sites are included on the primers, then integrated on the second chromosome by site-directed insertion using the phiC31 integrase and an attP landing site carrying recipient line, y[1] w[1118]; PBac{y-attP-9A} VK00018 (Bloomington Drosophila Stock Center #9736) (Groth et al., 2004).
Similar(59)
Towards this end we used a fly strain that carried multiple lacO tandem repeat binding sites for the LacI repressor stably integrated on the third chromosome as described in [36].
The irony is that had European economies had been as integrated on the eve of the First World War and plugged in with the communications we take for granted today, the current head of the Romanov family would have welcomed the world to the Sochi Olympics, not Vladimir Putin.
My football team was becoming integrated for the first time.
We conducted a comprehensive study of published and new embryological data comprising 15 turtle and eight tetrapod species belonging to other taxa, integrating for the first time data on the side-necked turtle clade.
And sometimes the right answer is to integrate everything on the first day, but sometimes it's not.
The compact design relies on a cooled housing, integrated into the first wall cooling system, and a calotte-shaped graphite anode.
In a very different approach, Christian Ospelkaus of Leibniz University, Hannover, Ger., and colleagues used a waveguide integrated on a microchip to produce the first microwave quantum gate that is, a logic gate for a quantum computer.
To complete the encoding, we constructed the first shadow by integrating all share1s, the second shadow by integrating all share2s, and so on.
On the other hand, integrating the second equation of system (4.2) over from to and over from to, respectively, we obtain (4.8).
We also observed that silencing can become PRG-1 independent using a second piRNA sensor construct integrated on a different chromosome (the piRNA mCherry sensor; Figure S5).
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