Development of a white clover integrated linkage map required matching and alignment of homoeologues between populations.
Of the 1743 loci mapped onto the white clover integrated linkage map, 951 showed sequence similarities to the ESTs or genome sequences of markers mapped onto the integrated linkage map of red clover.
Of significant interest are JaloEEP558 and BAT93, the two parental lines of a RILs population used to set up the integrated linkage map of P. vulgaris [ 29], and BAT93 and G19833 whose complete genomes have been sequenced [ 2].
Generally, large AFLP linkage groups were associated with linkage groups assigned to macrochromosomes (e.g. CJA01 – CJA07) while small AFLP groups were linked to integrated linkage groups assigned to microchromosomes (e.g. CJA09 – CJA09).
The resulting EST-SSR markers, integrated linkage map, and observed macrosynteny produced by this work will be valuable resources for genetic mapping, QTL analysis, and molecular breeding of white clover in the future.
Integrated linkage maps have been constructed in various plant and animal species, including insects.
Further, integrated linkage maps with different types of DNA markers were developed for many species of eucalypts.
Further, SSR markers were always preferred as framework markers for developing consensus linkage map, composite integrated linkage map and comparative map between the species (Garcia et al. [2011]; Hudson et al. [2012]).
An integrated linkage group composed of the 10 maize chromosomes was constructed for the four backcrosses with the 65 SSR markers.
When building an integrated linkage map for joint linkage mapping or joint association-linkage mapping, variation in recombination frequency among families is commonly observed in some chromosome regions [6].
We aimed to present an integrated linkage map, with relative marker positions and distances based on both sets of incompatible BI-markers linked together with MI, codominant AFLP and microsatellite markers.
