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For integration, worms were UV treated and integrated lines were outcrossed for at least 4 times.
Germ line expression of reporters appears favoured by chromosomal integration of fusion genes and no chromosomally integrated lines were generated for Promoterome fusions for one of these genes, mep-1.
Mos1 transposon or microparticle bombardment methods have been developed to create single- or low-copy chromosomal integrated lines.
Interestingly, in comparison to the corresponding extrachromosomal array lines, no extraneous expression was observed in any of the 135 chromosomally integrated lines suggesting that the position of insertion into the genome had no effect on reporter expression.
This revealed that each of the four Tau isoforms were expressed from the 68A site at similar levels, as expected (no significant differences were observed; Fig. 3C), but notably, expression from the 68A insertion site was approximately half that from four of the five randomly integrated lines we generated.
Integrated lines were backcrossed with N2 at least twice.
Similar(35)
Local integration overlay result shows a distribution of high-value traffic comprises with clusters of the settlement, it shows whereabouts habitants settled decision to choose on tranquil of low integrate lines in Local integration value.
(2) How public spaces related to this axis or other highly integrate lines?
The current archaeological approach integrates lines of evidence from archaeology, ethnography, ethnohistory, linguistics, biological anthropology, and native oral traditions.
As well as the largest public space in some 'Lan Na' historic cities, it directly attaches to high integrate lines that represent as urban axis core.
We chose to integrate lines of 99CGG and 0CGG with similar transgene copy numbers.
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