Sentence examples for integrated into the expression from inspiring English sources
At this point, two genes of the first round were integrated into the expression vector.
Then the PCR product was digested with KpnI (TaKaRa) and integrated into the pTD1 expression vector (SHIMADZU Biotech) [ 23] according to the manufacture's instruction.
Both 14C5 gene constructs were integrated into the pES33 mammalian expression vector and were co-expressed in the mammalian HEK293T cell line.
Briefly, murine IL-4 or murine IL-10 gene was obtained by RT-PCR, was then integrated into the respective eukaryotic expression plasmid pcDNA3.0, and the recombinant plasmid was finally confirmed by DNA sequencing.
One feels an aesthetic touch integrated into the environmental fabric through expression of social and spiritual bases.
In the in planta system, an HR template flanked by a nuclease recognition site is stably integrated into the plant genome; subsequent expression of nuclease simultaneously releases the HR template from the genome and induces a DSB at the target site.
The NICE system consists of a bacterial host with nisRK regulatory genes integrated into the chromosome and an expression vector carrying the gene of interest under the control of a nisA promoter [ 31].
Using BLI, cell tracking can be performed over long periods of time since the luciferase gene is stably integrated into the genome, and luciferase expression is preserved during proliferation or differentiation so that all cell progeny express luciferase without dilution [ 81].
According to our previous study, the copy number of the expression vectors integrated into the genome was assumed to be one (Ishida et al. 2000).
The majority of transformants will have a single copy of the expression vector integrated into the genome, and numerous clones will have to be screened to find high-copy transformants (Lin-Cereghino and Lin-Cereghino 2007).
In the last decades, optical reporter genes for both fluorescence (FLI) or bioluminescence imaging (BLI) have been extensively employed to image molecular pathways at the transcriptional level or integrated into the genome for long-term expression and for the development of transgenic animals [ 3– 5].
