Sentence examples for integrated fragment from inspiring English sources

The transgenic mice bred normally with the wild-type to give birth to offspring, some of which carried the integrated fragment).

Integration candidates were then analyzed by sequencing both strands of fragments obtained from PCR reactions using the candidates' genomic DNA as template and primers OAD476 and OAD477, which amplify a region that includes the entire integrated fragment as well as flanking sequences.

In this study, we demonstrated that the integrated fragment could be detected by PCR in the F4 mice, and also continuous downregulation of FPPS was detected in the heart of F2 and F3 mice, which indicated the stable transmission of the lentivirus-mediated silencing cassette in our model.

Genetic transfer between host and virulent phage genomes can occur; illegitimate recombination allows the integration of host DNA and if the integrated fragments carry genes that are useful or advantageous to the phage, or to hosts that the phage subsequently infect, they may be maintained in the genome.

To directly demonstrate repression of CD44 expression by miR-328, we integrated fragments of the CD44 3'-UTR containing the miR-328 target sequences into a luciferase report vector (pMIR-Report, Ambion; for structures and sequence see Figure S8).

Therefore, when they enter the nucleus, the mtDNAs in H. magnipapillata provide more sources of integrated fragments than those in N. vectensis and A. digitifera.

Furthermore, this hypothesis is supported by previous data, indicating that the viral integrated fragments might be reassembled to constitute the EsV-1 genome.

Furthermore, the genome of polydnavirus possesses remnants of transposon and retrovirus-like elements, such as the viral integrated fragments [ 38, 37].

If this turns out to be the case, it would be interesting to know whether the viral integrated fragments are replicated along with the EsV-1 genome or genetic exchange occurs between the viral genome and its integrated replica.

In addition, although some integrated fragments are not present as circular segments after the replication, some products translated from these fragments are necessary to achieve the replication of the circular segments [ 36].

This could explain the presence of single-stranded DNA regions in the EsV-1 genome [ 24] It can also be argued that these integrated fragments are ancient viral sequences that are degenerating, and the EsV-1 genome is present as an extra chromosome.