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This feature integrator integrates flag Is and flag IIs as shown in Figure 4.
The dis3-54 mutant was crossed with an S. pombe strain that carried the chromosomally integrated Flag-tagged Chp1 protein, which is a chromo-domain protein and a component of the RITS complex [33], [41].
For chromatin immunoprecipitation (ChIP) of condensin SMC2 subunit Cut14, we employed an S. pombe strain designated as Cut14-FLAG, which contains a chromosomally integrated FLAG-tagged cut14+ gene under the native promoter (Nakazawa et al. 2008).
The state flag of Mississippi integrated the Battle Flag as its canton, while the flag of Georgia, in its various permutations, included elements of both the Battle Flag and the Stars and Bars.
The cdc25-22 sthatn that contained the chromosomally integrated Mis17-FLAG was transformed with a plasmid carrying Myc-tagged N-Mis17 (N-Mis17-Myc) under the TET promoter.
That design integrated the Battle Flag as a canton on a field of white.
ES cells were stably integrated with NRBP1-Flag-MSCV after selection with neomycin.
For example, the MEDLINE search flagged 457 integrated prediction studies and 503 integrated intervention studies.
GFP or FLAG sequences were integrated at the 3' terminus of the GatewayR cassette for the production of epitope-tagged fusion proteins that will facilitate subsequent immunoprecipitation and coimmunoprecipitation analyses.
These data further confirm that FLAG-H2BE is integrated into the chromatin of olfactory neurons.
These results provide strong evidence that FLAG-H2BE is integrated into the chromatin of olfactory neurons.
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