Thus, we conclude that spikes have the potential to initiate at multiple domains and then to integrate downstream at branch-point nodes in the arbor.
Interestingly, also in Streptococcus agalactiae several mobile genetic elements have been found to be integrated downstream of rpsI [ 78], suggesting that this gene forms a hot spot for genomic diversity in different Gram-positive bacteria.
NEO was subsequently integrated downstream of the promoters of random BESs conferring resistance to G418.
ExoS233-419, ExoS233-453, ExoS233-453 sequences were amplified by PCR and integrated downstream the galactose-inducible GAL1-10 promoter in the yeast integrative plasmid vector YIplac211.
The methanol process for carbon dioxide removal is integrated downstream the existing desulfuration unit, and after a CO shift conversion unit.
Plug-flow conservation equations for the bulk fluid states are coupled with the discretized pellet equations, and the resulting differential-algebraic system is integrated downstream by an adaptive implicit algorithm [17,3].
The results showed that the reporter protein had an obvious increase when this element was integrated downstream of the polh promoter (Wang et al. 2009).
For gRNA vectors construction, gRNAs were designed using online CRISPR Design (http://crispr.mit.edu/), and the synthesized oligos were annealed with sticky terminus of HpaI and XbaI restriction site, and then integrated downstream U6 promoter in a middle vector PUG3 (U6-gRNA).
SGI1 was thus integrated downstream of the sodB gene coding for the iron superoxide dismutase and 208 bp upstream of the purR gene coding for the transcriptional repressor for purine nucleotide synthesis (Fig. 1B).
They are also the only countries with fully integrated downstream forestry processing facilities.
