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Moreover, the ecological success shown by the red strain suggests that this strain is the most suitable for integrated cultivation.
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By integrating cultivation strategies and dynamic metabolic profiling techniques using strain JSC4, this study demonstrated an innovative technology for practically and markedly enhancing microalgal lipid productivity and elucidated the details of carbon metabolism related to lipid biosynthesis.
When integrated with cultivation-based studies, the analysis of the SSU rRNA gene sequences as phylogenetic markers enabled rapid identification of the new gastrointestinal isolates, and illustrated the need for the reclassification of many species.
The systems-level impacts of integrating algae cultivation with wastewater treatment were found to significantly reduce environmental impact.
A Geodesign model is suggested for managing the coupled system that integrates algae cultivation and urban systems as "design for change".
To integrate rice cultivation in poverty and hunger reduction, upland farmers should be able to grow rice.
So far no cultivation integrated foam fractionation process for rhamnolipid production has been reported.
This led to an unforeseeable effective method to produce and in situ concentrate rhamnolipids via simple cultivation integrated foam fractionation using the heterologous production strain P. putida KT2440 containing a plasmid for mono-rhamnolipid production in a bioreactor.
In this article a cultivation integrated foam fractionation process for rhamnolipids in a bioreactor using P. putida KT2440 as a heterologous production strain is described with low biomass enrichment in the foamate giving the opportunity to remove highly concentrated rhamnolipids from the cultivation broth in situ.
It was previously shown that simple cultivation integrated foam fractionation is an effective tool for biosurfactant production e.g. of surfactin using Bacillus subtilis (Chen et al. 2006; Willenbacher et al. 2014, 2015) or HFBII using Saccharomyces cerevisiae (Winterburn et al. 2011).
However, cultivation integrated foam fractionation in rhamnolipid production was shown to be not feasible due to highly concentrated biomass in the foam leading to the necessity to develop solutions regarding cell retention e.g. using magnetic separation or cell recycling (Gruber 1991; Heyd et al. 2011; Küpper et al. 2013).
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