Sentence examples for integrated copy of from inspiring English sources

We inserted the GFP-encoding sequence at the predicted 3′ end of the crb-3 gene, and generated transgenic lines carrying an integrated copy of this construct by gamma-irradiation mediated integration of an extrachromosomal array.

Once we confirmed the functionality of both of the MosSCI-engineered duplications in vivo, we constructed a strain that has three copies of the cyb-3 gene: an endogenous cyb-3 located on chromosome V, dotSi100 integrated copy of cyb-3 located on chromosome II, and the cyb-3 integrated on chromosome IV (dotSi110 IV).

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To determine whether dis3-54 culturedltured at 20°C were arrested at a pre-anaphase stage or following the switch to anaphase, the dis3-54 mutation was combined with a chromosomally integrated copy of a GFP-tagged Cut2/securin gene under the control of its native promoter.

In order to understand the role of phosphorylation of FtsZ on its interaction with FtsQ, merodiploid strains lacking the chromosomal copy of ftsZ and carrying an integrated copy of the wild type His-ftsZMTB (M. smegmatis-F1) or its T343A mutant (M. smegmatis-F2), were generated.

RG37, a human SV40-immortalized fibroblast cell line, contains a single chromosomally integrated copy of a tandem repeat of two inactive GFP genes.

are derived from the HeLa cell line and stably express high levels of CD4, CXCR4, and CCR5 on the cell surface and have integrated copies of the luciferase and β-galactosidase genes under control of the HIV-1 promoter.

Therefore, we used pulsed-field gel electrophoresis (PFGE) to assess whether the specific attP fragment revealed by Southern blot hybridization represented integrated copies of SGI1 arranged in tandem or not.

In line with previous reports showing that HPV type 16 is found in almost all cases of HPV+ HNSCC, all FF HPV+ HNSCC samples tested in this study had integrated copies of HPV type 16.

All four Ad-transformed rat cell lines contained integrated copies of the respective transforming (E1) region and expressed the transforming E1A and E1B proteins ((K. Raska, personal communication and GEB, unpublished results).

Deletion of the endogenous metE and placement of a chromosomally integrated copy under the control of a modified cpcG2 promoter (PcpcG2Δ59) that is activated by CcaR results in light-inducible growth-rate modulation when cells are grown in M9 medium lacking methionine.