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The kinetic growth curves were calculated using the integrated confluence algorithm and the result was provided as the percentage of confluency.
Briefly, cells were plated into 96-well or 24-well plate and allowed to grow for 24 hours with or without subsequent transfection, before acquiring phase-contrast images and an integrated confluence metric as a surrogate for cell number every 4 hours by IncuCyte.
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In ECIS, cell layers are grown to confluence not on porous membranes but directly on integrated gold-film electrodes.
When the cultures reached confluence, CSC-pretreated C. albicans was seeded inside the upper chamber and integrated to the culture system.
Thus, this region appears at the confluence of conscious perception of object identity and location, making it a primary candidate for the integrated perception of a unified conscious scene or event (Oizumi et al., 2014).
Everything is integrated.
Acquisitions were poorly integrated.
"We are totally integrated.
RIL is vertically integrated.
They are all vertically integrated.
They have integrated very well.
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