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Exconjugates carrying the plasmid integrated by a single homologous recombination were selected on LB agar with Nf and Tc.
Artists have been using everyday materials (rope, newspapers, scraps of metal) for many years, but rarely have the commonplace and the exalted been so successfully integrated by a single artist.
By using the hybrid transceiver, where the transmitter and receiver parts can be integrated by a single system.
Moreover, considering the importance of the effect of extrinsic signals on the neural fate decision, it remains unclear whether the inhibition of extrinsic signals and activation of internal factors are regulated separately or are integrated by a single determinant.
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Therefore, vector pEMG_ΔPP5004 was co-integrated by a single crossover into the chromosome of P. putida KT2440 using tri-parental mating with E. coli HB101 as helper strain, as well as the donor strain E. coli CC118λpir pEMG_ΔPP5004.
The two sides were supposed to have been integrated by now into a single army — marching, living and fighting together for one Sudan.
After India became independent in 1947, the princely states and chiefships of Rajputana were integrated by stages into a single entity.
Growth can only be restored when the plasmid integrates by a single homologous crossover into the genome, which results in a functional selection marker and a copy containing both mutations.
In contrast, cells in which the plasmid has integrated by single crossover recombination grow faster and produce larger colonies, because all of the progeny carry a copy of catP.
At some regular interval, say, every month, links to posts that have been submitted by users are integrated into a single blog post by a volunteer blogger.
Specifically, transgenic parasites that have the targeting construct integrated by single-cross over recombination are first selected using one of a limited set of resistance markers and drug combinations[5], [6], [7], [8].
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com