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Design and control of recycle systems must be integrated at an early design stage.
The EhGI1 in isolate 05-545 integratedted at an asn tRNA after the iutA2 gene in the chromosome.
An I-Ppo I recognition site is present in the rRNA transgene construct (see Fig. 1A), therefore a single copy of the transgene integrated at an ectopic site would be cut by I-PpoI to yield flanking fragments too large to resolve on a 0.3% agarose gel (Fig. 1B).
Finally, in clone 3.3, which never showed expression of RFP, the pHRT plasmid was integrated at an interstitial site within the p-arm of another marker chromosome.
To screen for chromosome loss, we generated a mwh + Y chromosome into which an mwh + genomic rescue fragment is integrated at an attP docking site (see Materials and Methods).
The clones in which pHRT integration had occurred at a telomere were able to produce RFP after a variable number of cell divisions, whereas the clone in which pHRT had integrated at an interstitial site was never able to express RFP.
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As homology arm length was decreased, integration efficiency also decreased, and the oligonucleotides were integrated at a low efficiency of approximately 0.15% (Fig. 3B).
We need think about how enabling access to research is integrated at a user level.
The results showed that climate and energy policy needed to be properly integrated at a federal level, Grossman said.
The division included Indians from more than 50 tribes and was integrated at a time when blacks and Asians were in segregated units.
Financial assessment is integrated at a later stage to assess the economic viability of the system.
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