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We discovered that SaPIbov1 is integrated adjacent to the conserved genomic islet νSaα (Figure 1).
As controls, the KanMX marker was integrated adjacent to the WT ERG26 and ERO1 loci of the W303 background via identical strategy, resulting in AMY1013-1A and SCVY15-2D, respectively.
Accordingly, comparative sequence alignment revealed a 33 kbp contiguous region integrated adjacent to (but not disrupting) the tRNA-Ser gene that was unique to 08-5578 and encoding several putative bacteriophage-related coding sequences (designated ϕLMC1) (Fig. 4).
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We produced Scc4 fused to bacterial LacI in wild-type and chl4Δ strains carrying lacO repeats integrated adjacent (∼500 bp) to the centromere on chromosome IV (CEN4; Figures 1A and 1D).
The plant will be integrated with an existing facility adjacent to the site.
Furthermore, integrated viral sequences can activate oncogenes adjacent to the insertion site resulting in cancer.
Similarly, immunoelectron microscopy revealed that gold particles were associated with the vesicles adjacent to the basal bodies, while they were not integrated into the basal body microtubule structures.
In all vcrABC -containing strains except GT, the vcr-GI is located adjacent to the primary site of integration, ssrA, structural evidence that vcr-GIs are among the most recently integrated of the available Dehalococcoides ssrA-GIs.
The respective tyrosine recombinase from C. ulcerans shares 92% amino acid sequence identity with the integrase of the β-corynephage integrated in the genome of C. diphtheriae NCTC 13129 and is encoded adjacent to the tRNAArg gene.
As in wild-type (WT), centrosomes that are located adjacent to nuclei upon mitotic entry in kif9 null cells are integrated into the nuclear envelope and proceed through division (Fig. 1).
It is located adjacent to the north border of Bangkok and in recent years has been integrated into the metropolis.
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