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The method relies on homologous integrative transformation with a plasmid that can integrate in two orientations, only one of which will disrupt the target gene function.
These results indicated that SGI1 was able to integrate in two distinct attB sites in a single wild type S. Typhimurium LT2 transconjugant.
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One might say this was the central promise of Darwinism to account for both phylogenic continuity and adaptive differentiation by means of the same principles; or as Darwin puts it, to integrate in one theory the supposed opposition between Unity of Type and Conditions of Existence.
The SRL PAI island and the clc element are able to integrate in one or both identical attB sites of the host chromosome.
The data are directly integrated in two data integration centres, at the University of Greifswald and at the German Cancer Research Center (DKFZ), which will share the task of offering data collection services (including constant on-line support) to the 18 study centres.
Double-stranded cDNA was cloned in a Gateway Topo cloning vector and the library was swapped into the pVV214 yeast expression vector [ 20]. αSyn is expressed from the GAL1 promoter, either on the multi-copy plasmid pESC-HIS (Agilent Technologies) or integrated in two copies in the genome, via integrative vectors pRS403 and pRS405 (Stratagene).
Adsorbents BM1 and BM2 were integrated in two different protocols for the purification P. fluorescens GaDH.
The model was integrated in two experiments at a horizontal resolution of 2.8° × 2.8° with 32 levels from the surface to ∼60 km.
After optimisations this material was successfully integrated in two metal level interconnects with excellent electrical and reliability results.
The system components have been implemented and integrated in two major successful trials in metropolitan railway stations in London and in Paris, as part of a major EU-funded project (PRISMATICA).
The Navier Stokes equations and the transport equation of concentration are integrated in two dimensions by the finite volume method using the Euler Euler approach, with the help of numerical software PHOENICS; the turbulence in the liquid phase is described using the k ε model.
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