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The dietary sodium intake was calculated from the 3-day food record using the Software Avanutri®.
Based on these recorded data, daily feed intake was calculated and feeding behavior was evaluated.
Dietary caloric intake was calculated for 3 days before each measurement.
Total intake was calculated by adding the nutritional content of the oral supplement.
The contribution of each food item to the population's intake was calculated.
Total energy and macronutrient intake was calculated using a computerized nutrient analysis program.
During the experiment, daily voluntary feed intake of each animal was individually recorded and dry matter intake was calculated.
Energy-adjusted intake was calculated using the nutrient-density method [34].
The preference for sucrose over water (the ratio of sucrose to total fluid intake) was calculated.
Food intake was calculated for all mice prior to starting CR.
The preference for sucrose over water (the ratio of sucrose to total fluid intake) was calculated at all time points.
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