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Overall comparisons between the R1 R7 structures determined here with the same region in the intact vault particle (Tanaka et al, 2009) showed a good superimposition of the central domains, from R3 to R6, but different changes in the relative positioning of domains R1, R2 and to a lesser extent R7, affecting the relative curvature of the fragment.
Here, we examine previously uncharacterized putative MVP sequences against these structural criteria, enabling us to predict with improved certainty whether the mvp gene, or relics of it, is likely to be present in a given species and whether intact vault particles are likely to form.
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Intact vaults, purified from rat liver were crystallized in different crystal forms, basically identical to the ones described by Querol-Audi et al (2005) and Kato et al (2008).
This urchin MVP has 64% sequence identity with the rat, and intact vaults have been seen via cryo-electron microscopy (Stewart et al. 2005), but the urchin MVP does not have a crystal structure determined.
Here, we report the high resolution structures of a recombinant MVP fragment, containing the seven N-terminal domain repeats (R1 R7), in three different crystal forms and of the intact vaults from rat liver determined at 8 Å resolution.
For example, in rat liver cell lysate, ultracentrifugation of purified MVP shows 95% of the population as a high molecular weight form (Kedersha et al. 1991); and antibodies fractionate with intact vaults rather than with individual monomers in rat neural cells (Paspalas et al. 2009).
In applying cross-hatching as a symbol for structures with intact vaulted roofs, no attempt has been made to limit this to vaulting that was intact before restoration.
Electron microscopy of vault particles from a variety of species indicates that the intact vault structure is strikingly conserved.
The sacristy/library, the south transept chapels, the treasury, the reredorter undercroft and the lower floor of the abbot's house still have their vaults intact.
Far out of view, in a subbasement 20 feet below street level, the old Tiffany vault is still intact.
The original facade of terra cotta, limestone and brick has been left intact, and the bank's vault is now a fitness center.
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com