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To examine the forebrain system in intact preparations, we prepared whole-mount "hemi forebrains" at E11.5 E12.5, by making a cut along the diencephalic midline, such that each telencephalic hemisphere remains attached to a hemi-diencephalon.
Furthermore, we find that the brain state as accessed in ECoG recordings is more stable and reproducible between animals, with dura intact preparations.
Cardiac cycle reconstruction yielded flow change estimations in under 10 minutes in intact preparations.
Similarly, norhydroguaiaretic has not been tested on transporter currents in intact preparations.
Isolated WT preparations showed HRs similar to those reported in intact preparations on an earlier occasion (21).
In endothelial cells there remain uncertainties in the details of how Ca2+ signals are generated and maintained, especially in intact preparations.
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This study was designed to investigate gap junctions coupling in an integrated intact preparation and to test if KIR channels modulate resting membrane conductance in "in situ" endothelial cells (EC), and acetylcholine (ACh -evoked relACh -evokedthe relaxationof mesentheic aratry.
We know of no other intact preparation affording such minute control.
These differences are likely a result of examining neurons in different regions of brain and in a more intact preparation.
By performing these experiments in organotypic brain slice culture, we show that modulation of EphB expression/signaling in individual neurons is sufficient to direct dendritic spine formation even in a more intact preparation.
While genetic deletion of EphBs from mouse brain has been found to result in fewer dendritic spines [16] and excitatory synapses [22], less is known regarding how reduced EphB expression in individual neurons in a more intact preparation impacts dendritic protrusion length.
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