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Therefore, the import of Par17 is dependent on an intact potential across the inner mitochondrial membrane.
Mitochondria with an intact potential display JC-1 red fluorescing aggregates, whereas in mitochondria with disrupted potential JC-1 is monomeric and green fluorescent.
Self-renewal is one of the hallmarks of CSCs, which refers to the ability to form new stem cells with identical, and intact potential for proliferation, expansion, and differentiation.
Grade I: fused with remodeling and trabeculae present; grade II: graft intact, not fully remodeled and incorporated, and no lucency present; grade III: graft intact, potential lucency present at the top or bottom of the graft; grade IV: fusion absent with collapse/resorption of the graft.
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The later, red fluorescence indicates mitochondria with intact membrane potential and green fluorescence indicates de-energized mitochondria.
The actual state of ecosystems is compared to a natural baseline that is intact or potential natural state.
Recently, it has been demonstrated that MV accumulates in mitochondria through a carrier-mediated transport which requires intact membrane potential and, once inside, increases intramitochondrial O2- production [40].
Successful fusion of the inner mitochondrial membranes requires an intact membrane potential [26], indicating that the activity of RC complexes and ATP synthase should be maintained under these conditions.
To determine whether combined mutations in Rb and p130 led to further disruption of the mitochondrial network seen in Rb deficient myotubes [36], we used MitoTracker®, a live-cell probe that accumulates in mitochondria with intact membrane potential.
In contrast to fission, fusion requires an intact membrane potential (ΔΨm) and is thus selective.
The dye used in these experiments (MitoTracker) is only accumulated by mitochondria with intact membrane potential.
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