Until recently, facile methods to study these processes in intact, living cells have not existed.
Our laboratory has been devising new ways to image hydrogen sulfide in intact, living cells with spatial and temporal resolution.
The goal of research in the Hynes laboratory is to understand these processes both at the molecular level and also in the context of intact, living organisms.
Here, we present new, high-resolution optical measurements that directly map sound-evoked vibrations on to anatomical structures in the intact, living gerbil cochlea.
This review describes an experimental strategy, devised to study protein protein interactions in any intact living cells based on protein-fragment complementation assays.
The use of full-length bacterial luciferase and the measurement of real-time light emission from intact, living cells make the present system suitable to follow the short term activity of different inducers.
Observing this level of anatomical detail in intact, living cochleae is unprecedented: even the two layers of collagen fibers that make up the pectinate zone of the BM can be distinguished quite clearly in most images, the highly oriented fibers acting as strong scatterers of the infrared light.
The study of intact, living cells with optical spectroscopy offers the opportunity to observe cellular structure, organization and dynamics in a way that is not possible with traditional methods.
Stanford Medical School Professor Karl Deisseroth et al., have described optogenetics as a technology which "combines genetic targeting of specific neurons or proteins with optical technology for imaging or control of the targets within intact, living neural circuits".
Therefore, internal organs and structures can be visualized in the intact, living organism, facilitating multiple or continuous observations of dynamic processes.
Second, to assess whether this effect was due to a cytotoxic mechanism, anti-CD3 sTimulated T cells were stained for the membrane impermeant DNA dye, propidium iodide (PI) that is excluded from intact, living cells, and analyzed by flow cytometry.
