Normal brain function depends on intact interactions between multiple neuronal ensembles.
To exclude data from high-throughput experiments and only include expert-curated interactions from small-scale experiments we only use those DIP and IntAct interactions that come from experiments with less than 100 interactions per publication.
The EMBL-EBI search (38) uses MImerge and MIscore to provide non-redundant summary information about molecular interactions, selecting specifically IntAct interactions with a high score.
We filtered out IntAct interactions that were based on spoke expansion (connecting bait to all preys in the case of a co-complex involving more than two molecules).
These interactions were studied with mesenchymal cells, which are targets of cytokines (synoviocytes, osteoblasts, myoblasts), as well as with the respective organ samples (synovium, bone, muscle) in order to keep intact interactions as seen in vivo.
Sma3s provides the following biological descriptors associated with each sequence: Gene ontology (GO) terms, Swiss-Prot keywords and pathways, InterPro domains, and IntAct interactions (Uniprot, UniProt Knowledgebase Release 2012_04).
Similarly, putative PPIs were derived through corresponding protein sequences using PPIs from the Drosophila Interactions Database [ 23] and the IntAct molecular interaction database [ 24].
The IntAct protein protein interaction database (10) was queried for high-quality binary interactions for the 88 human proteins that have experimental support for peroxisomal location (IntAct database release 154).
The IntAct molecular interaction database provides such a curation platform for several other data resources interested in capturing experimentally derived interaction data, mainly, but not exclusively protein protein interaction evidences.
When the two fluorescent dyes bound to the probe are in close proximity (when the probe is intact), the interaction of the two dyes prevents the reporter dye from fluorescing.
