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Cross-reactivity studies were performed for P. denticola, P. oris and P. melaninogenica to insure specificity of the primers.
Carrying out the appropriate negative controls, enable one to insure specificity for the target RNA.
The PCR primer sequences are BLAST searched to insure specificity to this particular gene.
Dissociation curve and agarose-gel analysis of each primer set were used to insure specificity of the amplicon.
Subsequent samples were analyzed using the MSD/DS running in SIM mode, with monitoring of m/z 76 and 225 to insure specificity to JHIII.
To insure specificity of the RT-qPCR, an agarose gel electrophoresis was initially performed to verify whether a single PCR product was generated and then a melting curve was performed at the end of each RT-qPCR.
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To insure the specificity of respective H2A.Z and H3K27me3 or H3K4me3 fluorescence signals, control analyses were performed that omitted each primary antibody individually or both together.
Rates have risen sharply.Reinsurers insure insurers.
The consigner should insure cargo transportation insurance.
But we insure against it.
We even insure our lives.
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