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KIF2a/MCAK co-depletion, however, did not suppress a mitotic arrest caused by the presence of insufficient tension (monastrol treatment) or unattached kinetochores (nocodazole treatment; Figure 5A).
Correct tension development is crucial for healing to proceed properly: insufficient tension may impede the healing process, or even lead to chronic wounds, whereas excessive tension may contribute to scarring.
The SAC responds to unattached kinetochores or to sister-kinetochores with insufficient tension (measured as the distance between the two sister-kinetochores) that become transiently detached due to the kinase activity of Aurora-B (Foley and Kapoor, 2013).
Aurora-B inhibition does not overcome a SAC-dependent mitotic arrest caused by unattached kinetochores (nocodazole treatment), but overcomes a mitotic arrest caused by insufficient tension at sister-kinetochores in monopolar spindles (monastrol treatment), as Aurora-B inhibition stabilizes kinetochore microtubules and prevents loss of kinetochore microtubule attachment.
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Even on our best behaviour, bad or insufficient city planning creates tension.
What was so attractive at an audition was precisely what went wrong in performance: electrifying tension and insufficient control.
However, corresponding increases in viscous forces as a result of a reduction of interfacial tension were insufficient to initiate and maintain the displacement (recovery) of the heavy crude oil fraction during surfactant flushing.
In areas where tension is insufficient to keep vinculin in an extended active conformation, FAs become labile and disassemble.
The lack of sufficient and effective efforts in the early stages of a program can result in unstable, unclear and incomplete requirements, unclear roles and responsibilities within the program organization, insufficient planning, and unproductive tensions between program management and systems engineering.
This procedure is certainly insufficient to drain adequately a tension pneumoperitoneum but it helps to reduce the intra-abdominal pressure, which might cause an abdominal compartment syndrome.
Despite this aggressive approach, oxygenation remained insufficient: arterial oxygen saturation 65%; arterial oxygen tension 44 mmHg; arterial carbon dioxide tension 56 mmHg; and alveolar arterial oxygen difference 600 mmHg.
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