This was not likely to be due to insufficient time for differentiation since endogenous DG NPCs acquire NeuN expression 2 weeks, and Prox1 expression 1 week, after the expression of DCX [3], [34].
The low differentiation among regions (8% of total variation) can both be explained by levels of gene flow that have been large enough to maintain genetic similarity and/or by insufficient time for differentiation to arise because of recent foundation from a common source.
For example, if the Comoros islands were colonized relatively recently, there may have been insufficient time for differentiation to occur despite the absence of any ongoing gene flow (non-equilibrium population).
In old age, muscle satellite cells, osteoblasts, and macrophages can sometimes dysdifferentiate into cells with an incomplete, adipocyte-like phenotype, with lipid accumulation and expression of the fat cell-specific fatty acid-binding protein, aP2, and PPARγ (but insufficient PPARγ for differentiation into fully functional, insulin-responsive fat cells).
Below 0.4×106 cells/ml, SPIO-generated R2* signal was insufficient for reliable differentiation from defects seeded with unlabeled hBMSCs.
Intriguingly, even a combination of TGF-β treatment and the expression of a constitutively active form of STAT3 is insufficient for the full differentiation of Th17 cells (Zhou et al. 2007), indicating the presence of a yet to be identified factor for Th17 differentiation.
Routinely available structural MRI utilizing T2- and gadolinium-enhanced T1-weighted sequences has insufficient sensitivity and specificity for differentiation between recurrence and radionecrosis or PsP, due to their similar imaging patterns characterized by contrast-enhancing lesion(s) surrounded by edema [ 3, 4].
Early care and education is largely composed of very small businesses, reducing opportunities for cost reduction (Stoney, 2004), and Insufficient product differentiation.
The adipogenic differentiation protocol was modified based on an evaluation of different protocols for equine MSCs [ 36], as insufficient adipogenic differentiation of equine MSCs has been repeatedly reported after standard induction [ 26, 58].
Language provides more opportunities for differentiation.
