The knock-out of megalin expression in the kidneys of these megalinlox/lox; apoECre mice occurs in a mosaic pattern, with a considerable percentage of tubular cells expressing normal levels of megalin, presumably caused by insufficient expression of Cre recombinase.
In addition, the immaturity of the lung results in the insufficient expression of anti-proteases.
However, increasing xylan concentration to 30 g/l does not enhance the production of butanol and hydrogen due to insufficient expression of xylanase enzyme (27.5 U/mg).
However, these studies overlooked the problem that the spinosyn biosynthetic pathway was unbalanced: the expression of six genes in the spinosyn biosynthetic pathway was insufficient; Saccharopolyspora spinosa accumulated useless compounds because of insufficient expression of SpnK.
This technology permitted us to avoid the insufficient expression associated with more severe consequences, such as lethality, and provided advantages similar to those obtained with mutations generating hypomorphic phenotypes.
Therefore, we can rule out the possibility that the observed phenotype of dy3K/δE3 mice described below is due to insufficient expression of truncated LMα1 chain.
We conclude, therefore, that the inability to efficiently terminate IFN-α-induced signaling in a subset of BLs is due to insufficient expression of UBP43.
In this regard, NFAT5-deficient mice suffer a progressive atrophy of their kidney medulla due to an insufficient expression of osmoprotective gene products and NFAT5-deficient cells have reduced proliferative capacity and defective expression of cyclins when cultured in hypertonic media [9], [10] [12].
While it is likely that the U6atac mutant phenotype results from the combined influence of insufficient expression of several U12-type intron-containing genes and their downstream effects, our data suggests that disruption of the prohibitin complex functions may play a central role.
Those with an average Ct value greater than 30 were discarded because of insufficient expression.
