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In addition, new tags in the form of peptides that bind fluorescent ligands and quantum dots offer the prospect of overcoming some of the limitations of fluorescent proteins such as excessive size and insufficient brightness.
Despite recent advances in improving the efficiency of UCNPs, the insufficient brightness of UCNPs is still the major reason that limits their popularity in biology.
The insufficient brightness is aroused from two parts: (i) The first is the low extinction coefficient of UCNPs, which is an intrinsic feature of 4f 4f optical transitions in lanthanide ions.
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There was capacity for 18 more lamps per pylon if it was felt the brightness was insufficient for the game.
However, in most former studies, positive results can be explained by insufficient control of the relative brightness of the stimuli.
Moreover, when using traditional tools, designers are unable to directly experience some lighting phenomena that may affect the visual perception of the occupants, e.g., brightness, darkness, glare, or insufficient illumination.
Conventional NIR dyes often suffer from limitation of low brightness due to self-quenching, insufficient photo- and bioenvironmental stability, and small Stokes shift.
Merely blocking out the Sun's radiant disk was insufficient to view the comparatively dim corona because of the diffusion of the Sun's light by the atmosphere, whose brightness rendered the delicate corona invisible.
His brightness.
Insufficient emotion.
Infected brightness.
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com