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These aptamers have often shown insufficient affinity for biomedical applications.
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In the off state, there is no response to a changing proton concentration throughout the tested range, which indeed suggests that protons cannot compete with Ca2+ ions due to insufficient affinity of Sp for protons.
These data suggest that, although Yel1 contains a recognisable PH domain, its affinity for phosphoinositides is insufficient to allow the protein to stably associate with the plasma membrane.
Pretreatment of mice with infliximab, instead, did not block the cytotoxic effects of SM83 on ascites tumour cells (data not shown), probably owing to its lower affinity for mouse TNF or to an insufficient concentration.
Alternatively, it is possible that dilp2 is less efficiently transported from the CNS to other tissues or has lower affinity for InR, such that CNS-specific expression is insufficient to rescue the imaginal-disc-mediated growth and viability defects.
In such a situation, the peroxidase with higher affinity for H2O2 will utilise it and hence the H2O2 is insufficient or unavailable for HRP to react with.
However, we cannot rule out the possibility that the deletion of the last 15 residues causes such a drastic decrease in the affinity for eEF2 that even a 10-fold increase in eEF2 concentration is insufficient to allow detectable phosphorylation.
That is, their presumed affinity for Christian communities in the Mideast, be they in Palestine or Syria or where ever, is insufficient for them to overcome the imperial impulses of "Christian Zionism".
Meanwhile, his affinity for business deepened.
He has an affinity for calculated risk.
And an affinity for bargain hunting?
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com