During this period, BG samples were collected for glucose measurements (YSI 2300, Yellow Springs Instruments, Yellow Springs, OH) every 15 min from the beginning of a meal until 3 h after and every 2 h in the other periods.
Rectal temperature was measured at 1400 to nearest 0.1°C, with an interchangeable thermistor probe system (model 46TUC Tele-thermometer, Yellow Springs Instruments, Yellow Spring, OH Grosss composition, the activities of PA, PG, and PL and the concentrations of Na+ and K+ in the milk were determined essentially as described previously [ 22, 27, 29].
For instance, the current International Standards Organization standard (ISO 15197) requires that 95% of results from a glucose meter be within 20% (or 15 mg/dl for values <75 mg/dl) of results obtained from a reference measurement such as a central laboratory hexokinase method or a Yellow Springs Instrument (Yellow Springs Instruments, Yellow Springs, OH, USA).
Blood glucose was determined using a bedside glucose analyzer (Yellow Springs Instruments, Yellow Springs, CO, USA).
Blood samples (30 µl) were taken from the tail vein at 0, 75, 90, 105 and 120 min. The concentrations of glucose in plasma were analyzed with a glucose oxidase method (YSI 2700 Select; Yellow Springs Instruments, Yellow Springs, OH); free glycerol was measured calorimetrically (Randox Lab Ltd ,Oceanside, CA ).
Blood glucose concentrations were determined with a glucose analyzer YSI2300 (Yellow Springs Instruments, Yellow Springs, OH).
Plasma glucose was measured using a YSI glucose analyzer (Yellow Springs Instruments, Yellow Springs, OH).
Plasma glucose was measured by using an automated glucose analyzer (YSI, Yellow Springs Instruments, Yellow Springs, OH).
Plasma glucose was measured by glucose oxidase method using a glucose analyzer (YSI 2300; Yellow Springs Instruments, Yellow Springs, OH).
Glucose and lactate were measured in duplicate with a YSI 2300 analyzer (Yellow Springs Instruments, Yellow Springs, OH).
Plasma glucose concentrations were measured with a YSI Stat2300 (Yellow Springs Instruments, Yellow Springs, OH) and plasma insulin using the insulin ELISA assay (Invitrogen, UK).
