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Using µQuant Microplate Spectrophotometer (BioTek Instruments), samples were measured by the absorbance at 450 nm with a reference wave length of 690 nm within 30 min after adding the stop reagent.

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Where the concentrations of the analytes were outside the range of the instrument, samples were diluted into Nova buffer which contained per liter 6 g HEPES acid, 0.64 g LiCl, and 0.46 g LiOH.

On both instruments the samples were focused using 20× and 50× objectives.

Prior to the measurement in the MALDI-TOF instrument, the samples were allowed to dry on the target 10 to 15 min. The MALDI-TOF measurement of spotted peptide solutions was carried out on a 4800 MALDI TOF/TOF™ Analyzer.

In some cases, depending on separation quality and/or instrument performance, samples were run twice pooling both sets of data.

Zeta potential of the NPs was detected using laser Doppler anemometry (LDA; Zetasizer Nano ZS90, Malvern Instruments Ltd .. Samples were prepared in PBS and diluted 1 3 with deionized water to ensure that the measurements were performed under conditions of low ionic strength where the surface charge of the particles can be measured accurately.

Following HP application, the pouches were opened with autoclaved instruments and the samples were returned to individual agarose coated wells.

By adopting a designed surface adsorption instrument-GC, the samples were oxidized at low temperature through pulse method to investigate their oxygen adsorption under different temperatures.

Nitrogen (N2) adsorption desorption isotherms were measured at 77 K on a Quantachrome Autosorb-3B instrument after the samples were outgassed at 473 K in vacuum at least for 10 h prior to investigation.

In the instrument development, convenience samples were chosen as participants of the focus group.

After the forearm was instrumented, baseline blood samples were collected and normal saline was given intra-arterially for 15 min, at which point an infusion of regular insulin (Humulin; Eli Lilly, Indianapolis, IN) at 0.1 mU/kg/min (1 mL/min infusion rate) was started in the same line.

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