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Strengths of the study are the use of consistent methodology and survey instruments over the study period and the study sample size.
Values below the limit of detection were reported as the limit of detection divided by square root of 2. Serum creatinine (Cr) was measured using the modified kinetic Jaffe reaction on two different instruments over the study period: the Beckman Coulter Synchron LX20 (Beckman Coulter, Fullerton, California) in NHANES 2003 2007 [ 21], and the Beckman Coulter UniCel® DxC800 in NHANES 2008 10.
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In addition, although just the last HONOS and risk assessment were considered for the analyses, the time from that assessment to dying from suicide should be taken into account when drawing associations of causality since risk factors evaluated by those instruments may have changed over the study period.
The uncertainties in the extent estimates vary with sampling network coverage and instrument type, and generally decrease over the study period.
Over the study period, four instruments were used for methemoglobin testing at the clinic sites and home visits.
However, this instrument was not yet used in a consistent way over the study period to take these data in account for this analysis.
Unfortunately, the results of the study do not provide strong evidence to prefer one of the instruments over the other.
13705_2011_23_MOESM1_ESM.doc Additional file 1 Title: Survey instrument used in the study.
The great benefit of the CarerQoL-7D instrument over earlier studies that measured the burden on informal caregivers is the fact that it can measure positive dimensions as well as the burden, such as satisfaction and support received from others.
The instruments used to study weather over the modern time scale, their known error, their immediate environment, and their exposure have changed over the years, which must be considered when studying the climate of centuries past.
This discrepancy may relate to the small sample size, suggesting that this study was underpowered to show superiority of one instrument over the other.
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