The instrument was normalized to standard white tile provided with the instrument before performing analysis on cells grown on solid BMS media in Petri plates.
PCR were carried out for 45 cycles using LightCycler® 480 SYBR Green I Master (Roche Diagnostic GmbH, Mannheim, Germany) in the LightCycler 480 instrument and normalized to ABL for each sample.
PCR experiments were performed in triplicate using the Applied Biosystems 7500 Real Time PCR instrument and normalized to the expression of U6. 10 ng of total RNA was reverse transcribed using a miRNA-specific primer then 1 µL of RT product was subjected to real-time PCR with a miRNA-specific probe.
One, which simply used nominal L1b VFM vector data in the VFM instrument frame (release 0302 when available, otherwise release 0301) in place of the ASM-V data; the other, built in the same way, except that each L1b VFM vector datum (still in the VFM instrument frame) was systematically normalized by the synchronous ASM scalar datum to ensure an exact match of its norm with this scalar datum.
Luciferase activity was measured on an AutoLumat LB 9505c luminometer (Berthold Analytical Instruments, Nashua, Germany) and was normalized by β-galactosidase activity.
"With the recovery now firmly in place, we need to move in a calibrated manner in the direction of normalizing our policy instruments," said Duvvuri Subbarao, the governor of the Reserve Bank of India.
Hybridized arrays were scanned with a GenePix 4000B Microarray Scanner (Axon Instruments, Union City, CA) and LOWESS normalized TIFF images were analyzed using Spotfinder ver. 3.2.1 (JCVI).
Also, some progress has been made to normalize fragmentation energies across instruments and instrument types [40, 44, 45].
Luciferase activities were measured with the Clarity Luminescence Microplate Reader (Bio-Tek Instruments, Winooski, VE), and firefly luciferase activities were normalized to Renilla luciferase activities.
Relative quantification was performed on the LightCycler Instrument (Roche, Mannheim, Germany) using LightCycler FastStart DNA Master SYBR Green I and normalized to Histone 3A as a housekeeping gene.
