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The times when natural scientists could only rely on an acute sense of observation, pencil and paper to document biological diversity never seemed so distant, A new golden age of exploration of the Earth's biodiversity is powered by the latest generation of sequencing instruments and sequence analysis algorithms.
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Clustered v3 flow cells are loaded onto HiSeq 2000 instruments and sequenced on 100 bp paired-end, non-indexed runs.
Hence, although different instruments and sequences were used, methods were fairly similar in 1989 and 2004, making a substantial systematic bias in results in 2004 vs. 1989 unlikely.
Diluted libraries were clustered on two lanes (one library per lane) of a paired-end flowcell using cBot instrument and sequenced using HiSeq2000 sequencer with TruSeq SBS Kit v3-HS (Illumina, USA).
Diluted libraries were clustered on a paired-end flowcell using cBot instrument and sequenced using HiSeq2000 sequencer with TruSeq SBS Kit v3-HS (Illumina, USA), read length 101 from each end.
Diluted libraries were clustered on a paired-end flowcell using cBot instrument and sequenced in 101 cycles using HiSeq2000 sequencer with TruSeq SBS Kit v3-HS (Illumina, USA).
Resulting normalized libraries were loaded on the MiSeq instrument and sequenced using paired-end 150-bp sequencing reads39.
The loaded PTP was then inserted into the Genome Sequencer FLX instrument, and sequencing reagents were sequentially flowed over the plate.
Clusters were generated on-board a HiSeq 2500 (Illumina) instrument and sequencing performed using TruSeq Rapid SBS Kit reagents (Illumina, FC-402 4001, FC-402 4001).
Each library was randomly assigned to one lane of an Illumina Genome Analyzer II instrument and sequenced to a mean depth of 30 million 76-base paireadsds (±4.5 million reads s.d., Supplementary file 1A).
Approximately 5 μg of purified cDNA was sheared into small fragments via Covaris E210 Acoustic Focusing Instrument and sequenced in three-fourths 454 plate run on a 454 GS-FLX Titanium platform (Roche).
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