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To address this question, Dr. Stephen Vatner and colleagues performed another series of experiments using a similar instrumentation protocol in mongrel dogs that underwent aortic banding to produce a spectrum of cardiac abnormalities ranging from severe left ventricular hypertrophy to decompensated heart failure (Fig. 5) [ 22].
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This has led to changes in instrument design, instrumentation protocols, and manufacturing methods.
The HART protocol is one of a number of smart instrumentation protocols designed for collecting data from instruments, sensors, and actuators by digital communication techniques.
We want to emphasize that we are not proposing the JSON metadata model as a possible standard, because we think that a single standard format cannot be achieved with the diversity of tools, instrumentation, protocols that are used in the Medical Science as a whole, let alone the variety of disciplines involved.
We suggest that subtle differences of the hybridization law due to details of chip-manufacturing and/or handling of the chips upon preparation as well as evolving instrumentation and instrument protocols give rise to slightly biased expression data between different array types and/or different batches of chips of the same type.
Variance component analyses can be used to interpret existing measurements, design effective exposure studies, and determine whether the instrumentation and protocols are satisfactory.
Hybridization, washing, and scanning of arrays was performed using standard Affymetrix instrumentation and protocols for 11 micron, 169 format arrays.
We are currently designing instrumentation and protocols to enable visualization of in vivo administered HYPOX-1 and -2 in mouse models of retinal hypoxia.
One of the investigators, who had collected PA data in the ES a few years prior (8), provided the protocol, instrumentation, and data to allow a direct comparison.
Again, this disagreement can be easily understood in terms of the pronounced differences in the patient groups and in the applied experimental methods (e.g., sample collection, pre-concentration, GC MS instrumentation and measurement protocol) between the two studies.
Miriam Rademaker was responsible for the conceptualization and design of the research and for all 'hands on' aspects, including surgical instrumentation of animals, protocol execution, statistical analysis and interpretation of data, and graphics and paper generation.
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