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Fv/Fm, maximum quantum efficiency or maximum quantum yield of PSII, was calculated through the built-in instrument protocol.
Calibration was updated before each acquisition using a standard peptide mixture according to instrument protocol.
qRT PCR was performed on the StepOne Real-Time PCR System (Applied Biosystems) using the standard instrument protocol for the TaqMan Protein Assay.
Fmoc deprotection was performed using the default instrument protocol: 20% piperidine in dimethylformamide (1 × 30 s, 1 × 3 min) at 75 °C (35W microwave power).
Coupling of the Fmoc-amino acids was performed using the default instrument protocol: 5 equiv (relative to resin loading) of Fmoc amino acid and HCTU in DMF with activation in situ, using 10 equiv of DIPEA.
Coupling of the Fmoc-amino acids was performed using the default instrument protocol: 5 mol equiv (relative to resin loading) of Fmoc amino acid and HCTU in dimethylformamide (DMF) with activation in situ, using 10 mol equiv of diisopropylethylamine (DIPEA).
Similar(53)
We suggest that subtle differences of the hybridization law due to details of chip-manufacturing and/or handling of the chips upon preparation as well as evolving instrumentation and instrument protocols give rise to slightly biased expression data between different array types and/or different batches of chips of the same type.
MRI was performed using the same instrument, protocols, and conditions as in the phantom MRI study.
The instrument protocols were followed providing a standardized interview format across respondents.
OBI is similar in content, and in some cases parallels ECO, in that it includes a classification of different types of instrument, protocols, analyses, materials and so on.
In this study, we use multidimensional performance metrics generated by QuaMeter to evaluate the data from CPTAC Studies 1 and 5. CPTAC Study 1 sought to characterize the performance of a broad collection of mass spectrometers prior to any cross-site coordination of instrument protocols.
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