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These spectral libraries were analyzed with PECAN and Skyline was used to select optimal transitions and to design an instrument method for SRM analyses.
Field comparison experiments revealed that the relative error was 5.61% between the developed system and the oil disk method and 6.88% between the developed system and the laser instrument method.
For the analysis of unlabeled peptides, the instrument method consisted of a full MS scan (scan range 375 1550 m/z, with 30K FWHM resolution @ m/z = 400, target value 2×106, maximum ion injection time of 500 ms) followed by data-dependent CID scans of the six most intense precursor ions.
The instrument, method, and technician were the same as in the baseline assessment.
The instrument method was also the same with the previous report [ 23].
In total, 39 different instrument method combinations were used to measure SCr.
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Therefore, the reliability of diagnosing 'delusions in general' and the subcategory of 'bizarre delusions' was reviewed, including both structured interview and standardized instrument methods.
All of the instrument methods were set up in the data-dependent acquisition mode.
KSA helped design the strategy, designed the robotic instruments, developed the custom robotic instrument methods, and helped analyze the data.
For formulation of the questions and the construction of the instrument, methods outlined by Streiner and Norman [ 26], were followed.
Skyline creates transition lists and vendor-specific instrument methods that can be imported directly into instrument control software for MS instruments from several vendors.
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