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After thermal cycling, the instrument determined the allelic content of each sample in the plate by reading the generated fluorescence.
According to Barbosa et al [ 19], the children's self-perception about oral health is one global rating in CPQ11-14, and the association with the overall score of the instrument determined the validity of schoolchildren's responses.
After thermal cycling (40 cycles of denaturation at 95°C for 15 seconds, followed by annealing and extension at 60° for 1-minute), the instrument determined the allelic content of each sample by reading the generated fluorescence, as described [ 16].
The study instrument determined the extent to which students felt they engaged with SDL; the group size engaging in SDL activities; the skills they practised most and least in SDL (fixed options); possible factors influencing their engagement with SDL, (including preparing for an imminent clinical examination), and finally, their perceptions of SDL.
Cella et al (2001), who used diagnostic criteria instead of a dimensional instrument, determined a prevalence rate of cancer-related fatigue of 17% in patients who completed chemotherapy treatment, most of whom had breast cancer, based on meeting at least 2 of the 14 suggested criteria.
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The instrument determines volume (or mass) concentration using a volume conversion factor Cv.
The aim of the instrument determines the measurement properties, which should be assessed in the validation process.
The sociodemographic and medical characteristics of any sample used to develop or validate a PRO instrument determine its appropriateness for future clinical study settings.
In accordance with the proposal by Clark and Watson, the number of items in an instrument determines the sample size to be used in the psychometric testing.
A pilot study was conducted in March 2010 with 30 students from all fields to test the instrument, determine application time, and clarify possible questions from the students.
This software, coupled with the LightCycler instrument, determines the threshold cycle (Ct) representing the number of cycles in which the fluorescence intensity is significantly above the background fluorescence.
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