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The genomic DNAs were extracted and sequenced on a Roche GS Junior instrument, as previously described [ 25, 31, 32].
For imaging, each tube was placed in a custom, in-house imaging instrument as previously described [ 24].
The blood was processed through a prototype liquid biopsy cell sample preparation instrument as previously described [ 13].
1 The metal content of Bvu0220 and Bvu0222 was determined using an Elan DRC II ICP-MS instrument as previously described.
mRNA levels of IL-32α, IL-32nonα and MxA were assessed by real time RT-PCR using the LightCycler480 instrument, as previously described [ 17].
For evaluating the therapeutic blood flow recovery, we used a laser Doppler perfusion imaging (LDPI) analyser (Moor Instrument) as previously described (Lee et al, 2009).
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Analysis of the pressure-conductance data was performed using PVAN 3.2 software (Millar Instruments, Houston) as previously described [ 8, 23].
Analysis of the pressure-conductance data was performed using PVAN 3.2 software (Millar Instruments, Houston) as previously described [ 10, 20, 21, 23].
At that time the bottle was removed from the BACTEC instrument and processed as previously described.
Real-time PCR detection was performed in triplicate on the ABI Prism 7700 using a TaqMan instrument (Applied Biosystems, Tokyo, Japan) as previously described (Tomita et al, 2003).
Samples were labeled using Cy-labeled random primers (9mers) and then hybridized onto the corresponding microarray platform and scanned using a GenePix 4000B scanner (Axon Instruments) as previously described[33].
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