Sentence examples for instructions with three from inspiring English sources
Shotgun libraries were prepared using the TruSeq DNA LT Sample preparation Kit (Illumina, San Diego, CA, USA) following the manufacturer´s instructions with three exceptions.
The mRNA fragment library was sequenced on a SOLiD 4 platform (Life Technologies) according to the manufacturer's instructions, with three technical replicates (sample identifiers: HI1, HI2, HI3; group identifier: HI).
Multiplexed magnetic bead-based immunoassay kits (Milliplex #RECYTMAG-65 K, EMD Millipore, Billerica, MA) were used to detect IL-1β, TNF-α, IFN-γ, and MCP-1 following the manufacturer's instructions with three exceptions.
Anti-HEV IgG in all serum samples were examined with both the Genelabs and Wantai assay, according to manufacturer's instructions, with three negative and two positive control wells included on each plate.
A package, including cooking instructions, with four generous portions, sauce and a choice of sides like candied yams, collards or macaroni and cheese, is $80.
It can track harmonic instructions with zero static error and good compensation performance.
Similarly SB3500 has three cores, each capable to handle SIMD instructions with four threads.
Total RNA was extracted using Trizol (Invitrogen) according to the manufacturer's instructions with one modification; absolute ethanol was used in place of isopropanol.
Cathepsin activity was assessed using the CV-Cathepsin B Detection Kit (Enzo Life Sciences) according to the manufacturer's instructions with one exception; after the final wash step, the plate was read on an Infinite 200 plate reader (Tecan) for fluorescence (550ex/610em), as opposed to microscopy.
Labeled RNAs were then purified using Absolutely RNA® Nanoprep Kit (Stratagene) following manufacturer's instructions with two elution steps in a final volume of 25 μL.
We performed DNeasy extraction according to kit instructions, with two elutions with buffer AE (10 mM Tris, 0.5 mM EDTA, pH 9.0) of 50 μL each (100 μL total volume).
