DNA digestion, labelling and hybridization were performed according to the manufacturer's instructions, with minor modifications.
The SBS library was constructed according to manufacturer's (Illumina) instructions with minor modifications.
The membrane protein (MP) was prepared with Bacterial Membrane Extraction Kit (BestBio, Shanghai, China) according to the instructions with minor modification.
Transfections were conducted according to the manufacturer's instructions with minor modifications.
The hybridization, washing and scanning of Agilent arrays was performed following manufacturer's instructions with minor modifications.
107 A17 cells were assayed with Phospho-Protein Purification kit (Qiagen, Milano, Italy) closely following manufacturer's instructions, with minor modifications.
(ChIP) was carried out according to standard protocols using a ChIP-IT kit from Active Motif (Carlsbad, CA), and following the manufacture's instructions with minor modifications.
DNA from bat skin tissue samples (untreated and paraffin fixed) was extracted with the QIAamp DNA FFPE kit (Quiagen, Valencia, CA) per manufacturer's instructions with minor modifications.
Total RNA was extracted from microdissected cells by using the picopure RNA isolation kit (Molecular devices - Arcturus) following manufactures instructions with minor modifications as described below.
RNA from this pellet was then extracted using RNA Blood Kit 100 (BioRad™) following the manufacturer's instructions with minor changes described bellow.
At least 1 µg of RNA from each sample was retrotranscribed by SuperScriptIII™ (Invitrogen) in the presence of random hexamers, according to manifacturer's instructions, with minor modifications.
