It can track harmonic instructions with zero static error and good compensation performance.
Total RNA was extracted using Trizol (Invitrogen) according to the manufacturer's instructions with one modification; absolute ethanol was used in place of isopropanol.
Cathepsin activity was assessed using the CV-Cathepsin B Detection Kit (Enzo Life Sciences) according to the manufacturer's instructions with one exception; after the final wash step, the plate was read on an Infinite 200 plate reader (Tecan) for fluorescence (550ex/610em), as opposed to microscopy.
Shotgun libraries were prepared using the TruSeq DNA LT Sample preparation Kit (Illumina, San Diego, CA, USA) following the manufacturer´s instructions with three exceptions.
Labeled RNAs were then purified using Absolutely RNA® Nanoprep Kit (Stratagene) following manufacturer's instructions with two elution steps in a final volume of 25 μL.
Anti-HEV IgG in all serum samples were examined with both the Genelabs and Wantai assay, according to manufacturer's instructions, with three negative and two positive control wells included on each plate.
We performed DNeasy extraction according to kit instructions, with two elutions with buffer AE (10 mM Tris, 0.5 mM EDTA, pH 9.0) of 50 μL each (100 μL total volume).
After washing with PBS/BSA buffer, the protocol was followed according to the manufacturer's instructions with two modifications: the reactions occurred at room temperature, and the incubation times were duplicated, except after the addition of the color reagent.
Cytokine levels were analyzed in conditioned media from astrocytes, mono-, and cocultures using the (RayBiotech, Norcross, GA) Human Cytokine Antibody Array G Series 5 according to the manufacturer's instructions with two subarrays per sample.
