A more adequate design would involve simultaneously timing of the performance of the participant by the three assessors, but the task instructions being performed by one assessor would result in potential bias for the measurements of the other two assessors.
A reverse-transcription PCR using Expand Reverse Transcriptase according to manufacturers instructions was performed (all solutions and enzymes for PCR protocols were obtained from Roche).
For semi-quantitative RT-PCR (sqRT-PCR), Trizol isolated RNA was cleaned with the RNeasy Plant Mini Kit (Qiagen) and a DNase I digest following the manufacturers instructions was performed.
In order to correct any detected faults, third redundant execution of the instruction is performed.
The GPUs used for the analysis all have a warp size of 32, that is, a minimum of 32 identical operations per instruction is performed in a Single Instruction Multiple Data (SIMD) fashion [47].
Neither professional prophylaxis nor tooth-brushing instruction was performed before or during the experimental period.
The calibration procedures for both portable and laboratory-based metabolic carts were carried out according to the manufacturer's instructions and were performed daily to ensure uniformity in their measurements.
If the vancomycin and teicoplanin disc diffusion test according to the current version of the EUCAST methodology [ 10] displayed glycopeptide resistance, E tests for both substances following the manufacturer's instructions (bioMérieux) were performed.
According to the manufacturer's instructions, the assays were performed using a Model 680 Microplate Reader.
To determine relative DNA copy number changes of these loci, we performed QPCR using an ABI Prism 7500® Real time PCR cycler (Applied Biosystems, Foster City, CA) with standard QPCR conditions according to the manufacturer's instructions (all analyses were performed in triplicate).
