Sentence examples for instructions were determined by from inspiring English sources

This stored-program computer was an advance upon previous machines such as the Electronic Numerical Integrator and Computer (ENIAC), in which the program instructions were determined by the wiring of the machine.

The PCR products were purified with QIAquick Wizard PCR purification Kit (Promega) according to the manufacturer's instructions, and the sequences were determined by cycle sequencing using the Taq Dye Deoxy Terminator Cycle Sequencing kit (Applied Biosystems, HTDS, Tunisia) and fragment separation in an ABI PrismTM 3130 DNA sequencer (Applied Biosystems, HTDS, Tunisia).

Nuclear proteins were extracted from 1,5×107 T-cells using the Active Motif Nuclear Extract kit according to the manufacturer's instructions, total protein concentrations of the lysates were determined by Bradford assay (Bio-Rad).

cDNA purification was performed according to the manufacturer's instructions, and concentration and frequency of incorporation were determined by spectrophotometry.

Large-scale plasmids were extracted with the Endotoxin-Free Mega kit according to the manufacturer's instructions (Qiagen, Hilden, Germany), and the concentrations were determined by A260/A280 measurement.

sCD25 levels were determined by ELISA according to manufacturer instructions (BD Biosciences).

Levels of carbonylated proteins were determined by ELISA Enzo Life Sciencess) following manufacturer's instructions.

All the above parameters were determined by spectrophotometry according to the manufacturers' instructions (Lei et al. 2015).

Cytokine levels were determined by Bioplex according to the manufacturer's instructions (Bio-Rad).

Plasma IL-6 levels were determined by ELISA Invitrogen Corporationn), according to the manufacturer's instructions.

GSH and MDA levels were determined by HPLC according to the manufacturer's instructions (ImmuChrom GmbH Schimadzu Prominence, Kyoto, Japan).