Compliance with dietary instructions was assessed at all visits following visit 1 based on patient self-reporting.
Next, the credibility of the genital pain and sexual enjoyment instructions was assessed as a manipulation check.
Effects of CTOA and cue instruction were assessed by means of a two-way cue×CTOA anova.
The current methods of instruction were assessed together with the existing needs and problems encountered in the teaching learning process.
The cost per instruction is assessed by measuring the average current of the processor when it executes that instruction.
Secondly, the retention of the acquired knowledge through different methods of instruction was assessed only after two months.
Novices' ability to follow the instruction was assessed orally prior to the data collection.
Markers rs9652490 and rs11856808 were genotyped by TaqMan SNP Genotyping Assay following the manufacturer's instructions and results were assessed using the Applied Biosystems 7900 Fast Real-Time PCR System and SDS software (vs. 2.2.2) for allele calling.
Genomic DNA was isolated from each tumour sample using a Wizard SV Genomic Purification System (Promega, Southampton, UK) according to the manufacturer's instructions, and DNA concentrations were assessed using a Nanodrop spectrophotometer (Thermo Fisher Scientific, Loughborough, UK).
Immunohistochemistry was undertaken using the Novolink Polymer Detection as per manufacturer's instructions, and the following markers were assessed: Ki67, cleaved caspase-3 (quantitative assessment), ALDH1A1, CD133, CD26, Nanog, XRCC1, ERCC1, MLH1, MSH2, MSH6 (semi-quantitative assessment via a trained pathologist).
